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Transplantation of schistosome sporocysts between host snails: A video guide.

Gabriel MouahidAnne RognonRonaldo de Carvalho AugustoPatrick DriguezKathy GeyerShannon E KarinshakNelia LuvianoVictoria MannThomas QuackKate RawlinsonGeorge WendtChristoph GrunauHélène Moné
Published in: Wellcome open research (2018)
Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different Schistosoma species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host and give rise to human dwelling larvae. We present here a detailed step-by-step video instruction in English, French, Spanish and Portuguese that shows how these sporocysts can be manipulated and transferred from one snail to another. This procedure provides a technical basis for different types of ex vivo modifications, such as those used in functional genomics studies.
Keyphrases
  • epithelial mesenchymal transition
  • life cycle
  • endothelial cells
  • signaling pathway
  • induced pluripotent stem cells