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ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation.

Kristyna SmerkovaMarcela VlcnovskaSimona DostalovaVedran MilosavljevicPavel KopelTomas VaculovicSona KrizkovaMarketa VaculovicovaVojtech AdamRene Kizek
Published in: Nanobiomedicine (2015)
Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction. In this work, the combined advantages of magnetic particles-based isolation/purification with fluorescent properties of quantum dots (QDs) and antibodies targeted on specific recognition of DNA-cisplatin adducts are demonstrated. The formation of a complex between magnetic particles with surface modified by anti-dsDNA antibody, cisplatin-modified DNA and QDs labelled anti-cisplatin-modified DNA antibody was suggested and optimized.
Keyphrases
  • circulating tumor
  • cell free
  • single molecule
  • quantum dots
  • molecularly imprinted
  • nucleic acid
  • mass spectrometry
  • electronic health record