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Switchable and orthogonal gene expression control inside artificial cells by synthetic riboswitches.

Yuta IshiiKeisuke FukunagaAileen CooneyYohei YokobayashiTomoaki Matsuura
Published in: Chemical communications (Cambridge, England) (2024)
Here we report two novel synthetic riboswitches that respond to ASP2905 and theophylline and function in reconstituted cell-free protein synthesis (CFPS) system. We encapsulated the CFPS system as well as DNA-templated encoding reporter genes regulated by these orthogonal riboswitches inside liposomes, and achieved switchable and orthogonal control over gene expression by external stimulation with the cognate ligands.
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