A High-Throughput NanoBiT-Based Serological Assay Detects SARS-CoV-2 Seroconversion.
Taha AzadReza RezaeiRagunath SingaraveluTaylor R JamiesonMathieu J F CrupiAbera SurendranJoanna PoutouParisa TaklifiJuthaporn CowanDonald William CameronCarolina S IlkowPublished in: Nanomaterials (Basel, Switzerland) (2021)
High-throughput detection strategies for antibodies against SARS-CoV-2 in patients recovering from COVID-19, or in vaccinated individuals, are urgently required during this ongoing pandemic. Serological assays are the most widely used method to measure antibody responses in patients. However, most of the current methods lack the speed, stability, sensitivity, and specificity to be selected as a test for worldwide serosurveys. Here, we demonstrate a novel NanoBiT-based serological assay for fast and sensitive detection of SARS-CoV-2 RBD-specific antibodies in sera of COVID-19 patients. This assay can be done in high-throughput manner at 384 samples per hour and only requires a minimum of 5 μL of serum or 10 ng of antibody. The stability of our NanoBiT reporter in various temperatures (4-42 °C) and pH (4-12) settings suggests the assay will be able to withstand imperfect shipping and handling conditions for worldwide seroepidemiologic surveillance in the post-vaccination period of the pandemic. Our newly developed rapid assay is highly accessible and may facilitate a more cost-effective solution for seroconversion screening as vaccination efforts progress.
Keyphrases
- high throughput
- sars cov
- respiratory syndrome coronavirus
- end stage renal disease
- coronavirus disease
- sensitive detection
- single cell
- ejection fraction
- newly diagnosed
- chronic kidney disease
- prognostic factors
- public health
- peritoneal dialysis
- loop mediated isothermal amplification
- blood pressure
- crispr cas
- quality improvement
- label free
- patient reported