Toxoplasma gondii genotyping in AIDS patients using high resolution melt analysis (HRM analysis) in Khuzestan province, southwest Iran.
Jasem SakiReza ArjmandPublished in: Journal of parasitic diseases : official organ of the Indian Society for Parasitology (2023)
Toxoplasmosis is a parasitic zoonotic disease caused by Toxoplasma gondii , a common protozoan in the Apicomplexa phylum. Several studies in Iran have demonstrated the presence of the parasite in various hosts, but no data on T. gondii genotyping in HIV patients in Khuzestan, Southwest Iran, is available. One hundred of blood samples from AIDS patients were collected and tested by real-time PCR High Resolution Melting analyses for T.gondii detection and genotyping. T. gondii was discovered in 8 out of 100 (8%) AIDS patients with dominant Type I. This study suggest that HRM method demonstrated excellent discriminating ability for T. gondii , and AIDS patients should be tested for Toxoplasma detection and genotyping to prevent parasite pathogenicity.
Keyphrases
- end stage renal disease
- toxoplasma gondii
- high resolution
- ejection fraction
- newly diagnosed
- chronic kidney disease
- peritoneal dialysis
- prognostic factors
- genome wide
- gene expression
- dna methylation
- mass spectrometry
- patient reported outcomes
- human immunodeficiency virus
- south africa
- artificial intelligence
- sensitive detection
- quantum dots