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Identification of aceNKPs, a committed common progenitor population of the ILC1 and NK cell continuum.

Noé Rodríguez-RodríguezPaula A ClarkMayuri GogoiAna C F FerreiraBernhard KerscherAlastair CrispHelen E JolinJane E MurphyMeera SivasubramaniamLuisa PedroJennifer A WalkerMorgan W D HeycockJacqueline D ShieldsJillian L BarlowAndrew N J McKenzie
Published in: Proceedings of the National Academy of Sciences of the United States of America (2022)
The development of innate lymphoid cell (ILC) transcription factor reporter mice has shown a previously unexpected complexity in ILC hematopoiesis. Using novel polychromic mice to achieve higher phenotypic resolution, we have characterized bone marrow progenitors that are committed to the group 1 ILC lineage. These common ILC1/NK cell progenitors (ILC1/NKP), which we call "aceNKPs", are defined as lineage - Id2 + IL-7Rα + CD25 - α4β7 - NKG2A/C/E + Bcl11b - . In vitro, aceNKPs differentiate into group 1 ILCs, including NK-like cells that express Eomes without the requirement for IL-15, and produce IFN-γ and perforin upon IL-15 stimulation. Following reconstitution of Rag2 -/- Il2rg -/- hosts, aceNKPs give rise to a spectrum of mature ILC1/NK cells (regardless of their tissue location) that cannot be clearly segregated into the traditional ILC1 and NK subsets, suggesting that group 1 ILCs constitute a dynamic continuum of ILCs that can develop from a common progenitor. In addition, aceNKP-derived ILC1/NK cells effectively ameliorate tumor burden in a model of lung metastasis, where they acquired a cytotoxic NK cell phenotype. Our results identify the primary ILC1/NK progenitor that lacks ILC2 or ILC3 potential and is strictly committed to ILC1/NK cell production irrespective of tissue homing.
Keyphrases
  • nk cells
  • bone marrow
  • transcription factor
  • single cell
  • crispr cas
  • skeletal muscle
  • cell therapy
  • cell fate
  • peripheral blood
  • climate change
  • natural killer cells