A method for single-neuron chronic recording from the retina in awake mice.
Guosong HongTian-Ming FuMu QiaoRobert D ViverosXiao YangTao ZhouJung Min LeeHong-Gyu ParkJoshua R SanesCharles M LieberPublished in: Science (New York, N.Y.) (2018)
The retina, which processes visual information and sends it to the brain, is an excellent model for studying neural circuitry. It has been probed extensively ex vivo but has been refractory to chronic in vivo electrophysiology. We report a nonsurgical method to achieve chronically stable in vivo recordings from single retinal ganglion cells (RGCs) in awake mice. We developed a noncoaxial intravitreal injection scheme in which injected mesh electronics unrolls inside the eye and conformally coats the highly curved retina without compromising normal eye functions. The method allows 16-channel recordings from multiple types of RGCs with stable responses to visual stimuli for at least 2 weeks, and reveals circadian rhythms in RGC responses over multiple day/night cycles.
Keyphrases
- diabetic retinopathy
- optic nerve
- high fat diet induced
- induced apoptosis
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- optical coherence tomography
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- molecular dynamics simulations
- white matter
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- endoplasmic reticulum stress
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- blood brain barrier
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- cerebral ischemia
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