Parahydrogen hyperpolarization of minimally altered urine samples for sensitivity enhanced NMR metabolomics.
Kerti AusmeesNele ReimetsIndrek ReilePublished in: Chemical communications (Cambridge, England) (2022)
Parahydrogen hyperpolarization has been shown to enhance NMR sensitivity in urine analysis by several orders of magnitude if urine samples are prepared by solid phase extraction (SPE). We present a different approach, developed for minimal sample alteration before analysis. Removing SPE from the workflow allows to retain a wider range of metabolites and paves the way towards more universal hyperpolarized NMR metabolomics of low abundance metabolites.
Keyphrases
- solid phase extraction
- ms ms
- magnetic resonance
- high resolution
- liquid chromatography tandem mass spectrometry
- mass spectrometry
- high performance liquid chromatography
- solid state
- molecularly imprinted
- simultaneous determination
- tandem mass spectrometry
- gas chromatography mass spectrometry
- antibiotic resistance genes
- electronic health record