Cancer-related mRNA expression analysis using a novel flow cytometry-based assay.

Barbara DepreterJan PhilippéMagali MeulBarbara DenysKarl VandepoeleBarbara De MoerlooseTim Lammens

Published in: Cytometry. Part B, Clinical cytometry (2017)

PrimeFlow™ is a sensitive technique to investigate mRNA expressions, with high concordance to RT-qPCR. High (MYCN) and subtle (WT1) overexpressed mRNA targets can be quantified in heterogeneous and rare subpopulations e.g. LSCs. © 2017 International Clinical Cytometry Society.

Keyphrases

flow cytometry
binding protein
single cell
high throughput