Determination of 5-Formyluracil via Oxime-Based Nucleotide-Metal Coordination.

Kaiyuan ZhangBoshi FuGuangrong ZouWei YangShen YanTian TianXiang Zhou

Published in: Chembiochem : a European journal of chemical biology (2022)

2-(Aminooxy)-N-(quinolin-8-yl)acetamide was synthesized, and its ability to regulate activities of DNA polymerase was tested. In addition, we used the isothermal amplification technology to detect the content of 5-formyluracil sites in irradiated genomic DNA, which confirmed its capability for the detection of 5-formyluracil content in general samples. This study presents the first example of the determination of 5fU based on coordination chemistry.

Keyphrases

nucleic acid
circulating tumor
cell free
single molecule
solid phase extraction
molecularly imprinted
label free
gene expression
copy number
high resolution
drug discovery
genome wide