Anti-Inflammatory Effects of Catalpalactone Isolated from Catalpa ovata in LPS-Induced RAW264.7 Cells.
Hyo-Young KimAh-Reum HanYun-Seo KilEun Kyoung SeoChang Hyun JinPublished in: Molecules (Basel, Switzerland) (2019)
Catalpa ovata (Bignoniaceae) is widely distributed throughout Korea, China, and Japan. This study investigated the anti-inflammatory effects of catalpalactone isolated from C. ovata in lipopolysaccharide (LPS)-induced RAW264.7 cells. Catalpalactone significantly inhibited nitric oxide (NO) production and inducible NO synthase (iNOS) expression in LPS-induced RAW264.7 cells. The levels of cytokines such as interleukin-6 and tumor necrosis factor-α were reduced under catalpalactone exposure in LPS-induced RAW264.7 cells. Additionally, catalpalactone suppressed signal transducer and activator of transcription 1 (STAT-1) protein expression and interferon-β (IFN-β) production. Treatment with catalpalactone prevented interferon regulatory factor 3 (IRF3) and nuclear factor-κB (NF-κB) activation. Taken together, these results suggest that the anti-inflammatory effects of catalpalactone are associated with the suppression of NO production and iNOS expression through the inhibition of IRF3, NF-κB, and IFN-β/STAT-1 activation.
Keyphrases
- lps induced
- inflammatory response
- induced apoptosis
- nuclear factor
- dendritic cells
- cell cycle arrest
- nitric oxide
- toll like receptor
- signaling pathway
- anti inflammatory
- oxidative stress
- immune response
- cell proliferation
- endoplasmic reticulum stress
- transcription factor
- rheumatoid arthritis
- nitric oxide synthase
- cell death
- mass spectrometry
- pi k akt
- smoking cessation
- combination therapy