Dynamic chromatin architecture of the porcine adipose tissues with weight gain and loss.
Xuan TaoDanyang WangJiaman ZhangPengliang LiuYujie WangYu LinCan LiuZiyin HanKeren LongDiyan LiYu JiangGui Sen LiYu ZhangJingyi BaiXiaokai LiJing LiLu LuFanli KongXun WangHua LiZhiqing HuangJideng MaXiaolan FanLinyuan ShenLi ZhuYanzhi JiangGuoqing TangLianqiang CheBo ZengLiangpeng GeXuewei LiQianzi TangZhihua ZhangMingzhou LiPublished in: Nature communications (2023)
Using an adult female miniature pig model with diet-induced weight gain/weight loss, we investigated the regulatory mechanisms of three-dimensional (3D) genome architecture in adipose tissues (ATs) associated with obesity. We generated 249 high-resolution in situ Hi-C chromatin contact maps of subcutaneous AT and three visceral ATs, analyzing transcriptomic and chromatin architectural changes under different nutritional treatments. We find that chromatin architecture remodeling underpins transcriptomic divergence in ATs, potentially linked to metabolic risks in obesity development. Analysis of chromatin architecture among subcutaneous ATs of different mammals suggests the presence of transcriptional regulatory divergence that could explain phenotypic, physiological, and functional differences in ATs. Regulatory element conservation analysis in pigs and humans reveals similarities in the regulatory circuitry of genes responsible for the obesity phenotype and identified non-conserved elements in species-specific gene sets that underpin AT specialization. This work provides a data-rich tool for discovering obesity-related regulatory elements in humans and pigs.
Keyphrases
- weight gain
- transcription factor
- weight loss
- genome wide
- gene expression
- body mass index
- genome wide identification
- birth weight
- insulin resistance
- dna damage
- bariatric surgery
- high resolution
- metabolic syndrome
- roux en y gastric bypass
- type diabetes
- adipose tissue
- dna methylation
- single cell
- copy number
- rna seq
- gastric bypass
- skeletal muscle
- physical activity
- single molecule
- human health
- glycemic control
- obese patients
- mass spectrometry
- heat stress
- machine learning
- gestational age
- oxidative stress