In vitro display evolution of unnatural peptides spontaneously cyclized via intramolecular nucleophilic aromatic substitutions.

Takumi YokoyamaTakehiro AndoYukio TakamoriDaisuke FujiMasashi SatoSanthana VediMizuki YamamotoTakashi Kawakami

Published in: Chemical communications (Cambridge, England) (2022)

We report novel, ribosomally incorporatable, and intramolecularly cysteine-reactive fluorobenzoic acid-derived linkers for SELEX of mRNA-displayed unnatural peptides, which spontaneously cyclized via intramolecular nucleophilic aromatic substitutions forming thioethers. With this strategy we identified several novel PCSK9-binding peptides.

Keyphrases

amino acid
energy transfer
binding protein
transcription factor