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Multimodal profiling reveals site-specific adaptation and tissue residency hallmarks of γδ T cells across organs in mice.

Anastasia du HalgouetKerstin BruderNina PeltokangasAurelie Darbois-DelahousseDavid ObwegsMarion SalouRobert ThimmeMaike HofmannOlivier Lantz Sagar
Published in: Nature immunology (2024)
γδ T cells perform heterogeneous functions in homeostasis and disease across tissues. However, it is unclear whether these roles correspond to distinct γδ subsets or to a homogeneous population of cells exerting context-dependent functions. Here, by cross-organ multimodal single-cell profiling, we reveal that various mouse tissues harbor unique site-adapted γδ subsets. Epidermal and intestinal intraepithelial γδ T cells are transcriptionally homogeneous and exhibit epigenetic hallmarks of functional diversity. Through parabiosis experiments, we uncovered cellular states associated with cytotoxicity, innate-like rapid interferon-γ production and tissue repair functions displaying tissue residency hallmarks. Notably, our observations add nuance to the link between interleukin-17-producing γδ T cells and tissue residency. Moreover, transcriptional programs associated with tissue-resident γδ T cells are analogous to those of CD8 + tissue-resident memory T cells. Altogether, this study provides a multimodal landscape of tissue-adapted γδ T cells, revealing heterogeneity, lineage relationships and their tissue residency program.
Keyphrases
  • single cell
  • gene expression
  • rna seq
  • type diabetes
  • pain management
  • patient safety
  • oxidative stress
  • adipose tissue
  • chronic pain
  • dna methylation
  • high throughput
  • signaling pathway
  • peripheral blood
  • wild type