Multiphoton Activation of Photo-Strain-Promoted Azide Alkyne Cycloaddition "Click" Reagents Enables in Situ Labeling with Submicrometer Resolution.
Christopher D McNittHazel ChengSusanne UllrichVladimir V PopikMatthew BjerknesPublished in: Journal of the American Chemical Society (2017)
Irradiation of cyclopropenone-masked dibenzocyclooctynes with near-infrared pulses from a femtosecond laser triggers photodecarbonylation via nonresonant two- or three-photon excitation. Multiphoton-generated cyclooctynes undergo a SPAAC reaction with organic azides, yielding the expected triazoles. Multiphoton-triggered SPAAC (MP-SPAAC) enables high resolution 3-D photoclick derivatization of hydrogels and tissues.
Keyphrases
- high resolution
- ms ms
- drug delivery
- gene expression
- high performance liquid chromatography
- tandem mass spectrometry
- mass spectrometry
- liquid chromatography tandem mass spectrometry
- simultaneous determination
- liquid chromatography
- drug release
- hyaluronic acid
- living cells
- wound healing
- energy transfer
- tissue engineering
- radiation therapy
- water soluble
- quantum dots
- visible light