TGF-β Activated Kinase 1 (TAK1) Is Activated in Microglia After Experimental Epilepsy and Contributes to Epileptogenesis.
Dilaware KhanPeter BednerJulia MüllerFabienne LülsbergLukas HenningMarco PrinzChristian SteinhäuserSajjad MuhammadPublished in: Molecular neurobiology (2023)
Increasing evidence suggests that inflammation promotes epileptogenesis. TAK1 is a central enzyme in the upstream pathway of NF-κB and is known to play a central role in promoting neuroinflammation in neurodegenerative diseases. Here, we investigated the cellular role of TAK1 in experimental epilepsy. C57Bl6 and transgenic mice with inducible and microglia-specific deletion of Tak1 (Cx3cr1 CreER :Tak1 fl/fl ) were subjected to the unilateral intracortical kainate mouse model of temporal lobe epilepsy (TLE). Immunohistochemical staining was performed to quantify different cell populations. The epileptic activity was monitored by continuous telemetric electroencephalogram (EEG) recordings over a period of 4 weeks. The results show that TAK1 was activated predominantly in microglia at an early stage of kainate-induced epileptogenesis. Tak1 deletion in microglia resulted in reduced hippocampal reactive microgliosis and a significant decrease in chronic epileptic activity. Overall, our data suggest that TAK1-dependent microglial activation contributes to the pathogenesis of chronic epilepsy.
Keyphrases
- temporal lobe epilepsy
- inflammatory response
- neuropathic pain
- early stage
- mouse model
- lps induced
- lipopolysaccharide induced
- single cell
- spinal cord injury
- immune response
- spinal cord
- high resolution
- tyrosine kinase
- radiation therapy
- high glucose
- transforming growth factor
- electronic health record
- atomic force microscopy
- brain injury
- toll like receptor
- diabetic rats
- stress induced