Purification of active recombinant human histone deacetylase 1 (HDAC1) overexpressed in Escherichia coli.

Alessandra StefanNatalia CalonghiFabrizio SchipaniFabrizio Dal PiazGiorgio SartorAlejandro Hochkoeppler

Published in: Biotechnology letters (2018)

We described a procedure for the overexpression in E. coli of recombinant HDAC1, the purification of which in active form can be successfully performed, although yielding an octameric aggregate.

Keyphrases

histone deacetylase
recombinant human
escherichia coli
cell proliferation
minimally invasive
biofilm formation
cell free
cystic fibrosis
staphylococcus aureus