Comparative evaluation of three real-time polymerase chain reaction assays to detect Myxobolus cerebralis.
Wade CavenderChristine SwanSkylar WolfDanielle Van VlietAlison Aceves JohnsonAnna ForestRobert ShieldsThomas LochChristopher K KnuppJohn DrennanGavin GlenneySascha L HallettJoe MarcinoAimee ReedPublished in: Journal of aquatic animal health (2024)
The "fit for purpose" approach and preliminary completion of the World Organization for Animal Health validation pathway demonstrate that the K18S assay provides an alternate method for Mc testing. This work provides the foundation for acceptance of the K18S assay into the AFS-FHS Blue Book as a standardized test procedure for Mc.