Comparison between core-shell and totally porous particle stationary phases for fast and green LC determination of five hepatitis-C antiviral drugs.
Adel Ehab IbrahimHisham HashemMagda ElhenaweeHanaa SalehPublished in: Journal of separation science (2018)
The performances of core-shell 2.7 μm and fully porous sub-2 μm particles packed in narrow diameter columns were compared under the same chromatographic conditions. The stationary phases were compared for fast separation and determination of five new antiviral drugs; daclatasvir, sofosbuvir, velpatasvir, simeprevir, and ledipasvir. The gradient elution was done using ethanol as green organic modifier, which is more environmentally friendly. Although both columns provided very good resolution of the five drugs, core-shell particles had proven to be of better efficiency. Under gradient elution conditions, core-shell particles exhibited faster elution, better peak shape, and enhanced resolution adding to lower system backpressure. The column backpressure on sub-2 μm particles was more than twice that on core-shell particles. This gives a chance to use conventional high-performance liquid chromatography conditions without needing special instrumentation as that required for ultra-high performance liquid chromatography. The method was validated for determination of the five drugs by gradient elution using mobile phase composed of organic modifier ethanol and aqueous part containing 0.75 g sodium octane sufonate and 3.0 g sodium dihydrogen phosphate per liter at pH of 6.15. Detection was done using UV-detector set at 210 nm. The linearity, accuracy, and precision were found very good within the concentration range of 2-200 μg/mL.
Keyphrases
- liquid chromatography
- solid phase extraction
- tandem mass spectrometry
- high performance liquid chromatography
- ultra high performance liquid chromatography
- simultaneous determination
- mass spectrometry
- high resolution mass spectrometry
- molecularly imprinted
- gas chromatography
- hepatitis c virus
- drug induced
- single molecule
- magnetic resonance imaging
- high resolution
- low cost
- photodynamic therapy
- quantum dots
- ionic liquid
- sensitive detection
- magnetic resonance
- computed tomography
- real time pcr
- metal organic framework