Neutralization assay with SARS-CoV-1 and SARS-CoV-2 spike pseudotyped murine leukemia virions.

Yue ZhengErin T LarragoiteElizabeth S C P WilliamsJuan LamaIsabel CisnerosJulio C DelgadoPatricia SlevJenna RychertEmily A InnisMayte CoirasMatthew T RondinaAdam M SpivakVicente Planelles

Published in: Virology journal (2021)

SARS-CoV-2 spike/MLV pseudovirions provide a practical means of assessing neutralizing activity of antibodies in serum or plasma from infected patients under laboratory conditions consistent with biocontainment level 2. This assay offers promise also in evaluating immunogenicity of spike glycoprotein-based candidate vaccines in the near future.

Keyphrases

sars cov
respiratory syndrome coronavirus
high throughput
acute myeloid leukemia
bone marrow
machine learning
coronavirus disease
dengue virus
single cell