Improved Plasmodium falciparum dilution cloning through efficient quantification of parasite numbers and c-SNARF detection.
Tatiane Macedo-SilvaSanjay A DesaiGerhard WunderlichPublished in: Malaria journal (2021)
The combined use of flow cytometry for precise dilution and the c-SNARF method for detection of growth improves limiting dilution cloning of P. falciparum. This simple approach saves time, is scalable, and maximizes identification of desired parasite clones. It will facilitate DNA transfection studies and isolation of parasite clones from ex vivo blood samples.
Keyphrases
- plasmodium falciparum
- flow cytometry
- liquid chromatography tandem mass spectrometry
- liquid chromatography
- gas chromatography
- loop mediated isothermal amplification
- real time pcr
- label free
- circulating tumor
- mass spectrometry
- toxoplasma gondii
- tandem mass spectrometry
- trypanosoma cruzi
- case control
- solid phase extraction
- high resolution
- circulating tumor cells