Synthesis of hyaluronic acid hydrogels by crosslinking the mixture of high-molecular-weight hyaluronic acid and low-molecular-weight hyaluronic acid with 1,4-butanediol diglycidyl ether.

High molecular weight hyaluronic acid (HMW-HA) and low molecular weight hyaluronic acid (LMW-HA) were mixed at different ratios and cross-linked with 1,4-butanediol diglycidyl ether (BDDE) to prepare five hyaluronic acid hydrogels A-E. Enzymolysis stability, swelling rate, crosslinking degree, rheological characteristics, BDDE residual rate, surface microstructure, and cytotoxicity of different hydrogels were investigated. The results showed that hydrogel B obtained by 10% HA (w/v, HMW-HA and LMW-HA having a mass ratio of 4 : 1) crosslinking with 1% BDDE (v/v) had stronger in vitro anti-degradation ability, better mechanical properties and lower cytotoxicity than those prepared by mixing in different proportions. Hydrogel B has potential applications in regenerative medicine and tissue engineering.