Delivery of Active Peptides by Self-Healing, Biocompatible and Supramolecular Hydrogels.
Seyedeh Rojin Shariati PourSara OddisMarianna BarbalinardoPaolo RavarinoMassimiliano CavalliniJessica FioriDemetra GiuriClaudia TomasiniPublished in: Molecules (Basel, Switzerland) (2023)
Supramolecular and biocompatible hydrogels with a tunable pH ranging from 5.5 to 7.6 lead to a wide variety of formulations useful for many different topical applications compatible with the skin pH. An in vitro viability/cytotoxicity test of the gel components demonstrated that they are non-toxic, as the cells continue to proliferate after 48 h. An analysis of the mechanical properties demonstrates that the hydrogels have moderate strength and an excellent linear viscoelastic range with the absence of a proper breaking point, confirmed with thixotropy experiments. Two cosmetic active peptides (Trifluoroacetyl tripeptide-2 and Palmitoyl tripeptide-5) were successfully added to the hydrogels and their transdermal permeation was analysed with Franz diffusion cells. The liquid chromatography-mass spectrometry (HPLC-MS) analyses of the withdrawn samples from the receiving solutions showed that Trifluoroacetyl tripeptide-2 permeated in a considerable amount while almost no transdermal permeation of Palmitoyl tripeptide-5 was observed.
Keyphrases
- mass spectrometry
- wound healing
- drug release
- liquid chromatography
- hyaluronic acid
- drug delivery
- induced apoptosis
- extracellular matrix
- cell cycle arrest
- tissue engineering
- ms ms
- tandem mass spectrometry
- simultaneous determination
- high performance liquid chromatography
- ionic liquid
- high resolution mass spectrometry
- capillary electrophoresis
- cell death
- multiple sclerosis
- oxidative stress
- cell proliferation
- water soluble