Login / Signup

An unusual variant glycophorin expressing protease-resistant M antigen encoded by the GYPB-E(2-4)-B hybrid gene.

Hatsue TsuneyamaKazumi IsaNaoko Watanabe-OkochiKenichi OgasawaraMakoto UchikawaMasahiro Satake
Published in: Vox sanguinis (2020)
Serological tests and immunoblotting revealed that 103 of the 193 009 individuals (0·0534%) expressed protease-resistant M-active glycophorin having a molecular weight of 20 kDa. All the 103 individuals were S+ s- or S- s+. When reticulocyte mRNA from the individuals with M-active glycophorin (20 kDa) was examined by PCR and cloning followed by sequencing, a novel GYPE-B hybrid transcript was identified. Long-range PCR and sequencing using genomic DNA revealed that the individuals had a GYPB-E(2-4)-B hybrid gene. This hybrid gene was predicted to encode a 59-amino-acid mature glycoprotein that expresses no S or s antigens CONCLUSIONS: The prevalence of the M-active glycophorin (20 kDa) in the Japanese population is 0·0534%. This glycophorin is predicted to be a 59 amino acids polypeptide encoded by the novel GYPB-E(2-4)-B hybrid gene.
Keyphrases
  • copy number
  • amino acid
  • single cell
  • genome wide
  • genome wide identification
  • heat shock protein
  • risk factors
  • gene expression
  • dendritic cells
  • single molecule
  • real time pcr