Nanozyme-based dual-signal sensing system for colorimetric and photothermal detection of AChE activity in the blood of liver-injured mice.
Chang HeZhenyi KeKai LiuJiasheng PengQinghui YangLixiang WangGuangfu FengJun FangPublished in: Analytical and bioanalytical chemistry (2023)
Acetylcholinesterase (AChE), a crucial enzyme related to liver function, is involved in numerous physiological processes such as neurotransmission and muscular contraction. The currently reported techniques for detecting AChE mainly rely on a single signal output, limiting their high-accuracy quantification. The few reported dual-signal assays are challenging to implement in dual-signal point-of-care testing (POCT) because of the need for large instruments, costly modifications, and trained operators. Herein, we report a colorimetric and photothermal dual-signal POCT sensing platform based on CeO 2 -TMB (3,3',5,5'-tetramethylbenzidine) for the visualization of AChE activity in liver-injured mice. The method compensates for the false positives of a single signal and realizes the rapid, low-cost portable detection of AChE. More importantly, the CeO 2 -TMB sensing platform enables the diagnosis of liver injury and provides an effective tool for studying liver disease in basic medicine and clinical applications. Rapid colorimetric and photothermal biosensor for sensitive detection of acetylcholinesterase (I) and acetylcholinesterase levels in mouse serum (II).
Keyphrases
- sensitive detection
- loop mediated isothermal amplification
- gold nanoparticles
- liver injury
- low cost
- quantum dots
- photodynamic therapy
- label free
- drug induced
- drug delivery
- cancer therapy
- high throughput
- hydrogen peroxide
- nitric oxide
- high fat diet induced
- adipose tissue
- fluorescent probe
- resistance training
- body composition
- aqueous solution
- real time pcr