Briaviolides K-N, New Briarane-Type Diterpenoids from Cultured Octocoral Briareum violaceum.
Jing-Hao XuKuei-Hung LaiYin-Di SuYu-Chia ChangBo-Rong PengZhi-Hong WenPing-Jyun SungPublished in: Marine drugs (2018)
Four new briarane diterpenoids, briaviolides K-N (1-4), have been obtained from the cultured-type octocoral Briareum violaceum. Using a spectroscopic approach, the structures of briaranes 1-4 were identified. This study employed an in vitro model of lipopolysaccharide (LPS)-induced inflammation in the murine macrophage RAW 264.7 cell line, and found that among the four briaranes, briarane 2 possessed anti-inflammatory activity against inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expressions in cells. In addition, principal component analysis using the chemical global positioning system (ChemGPS) for natural products (ChemGPS-NP) was employed in order to analyze the structure-activity relationship (SAR), and the results indicated that the ring conformation of the compound has a leading role in suppressing the expressions of pro-inflammatory iNOS and COX-2 proteins in macrophages.
Keyphrases
- nitric oxide synthase
- lps induced
- nitric oxide
- inflammatory response
- structure activity relationship
- induced apoptosis
- endothelial cells
- oxidative stress
- cell cycle arrest
- signaling pathway
- toll like receptor
- molecular docking
- adipose tissue
- high resolution
- molecular dynamics simulations
- endoplasmic reticulum stress
- immune response
- amino acid