G-quadruplex DNA inhibits unwinding activity but promotes liquid-liquid phase separation by the DEAD-box helicase Ded1p.

Jun GaoZhaofeng GaoAndrea A PutnamAlicia K ByrdSarah L VenusJohn C MareckiAndrea D EdwardsHaley M LoweEckhard JankowskyKevin D Raney

Published in: Chemical communications (Cambridge, England) (2021)

G-quadruplex DNA interacts with the N-terminal intrinsically disordered domain of the DEAD-box helicase Ded1p, diminishing RNA unwinding activity but enhancing liquid-liquid phase separation of Ded1p in vitro and in cells. The data highlight multifaceted effects of quadruplex DNA on an enzyme with intrinsically disordered domains.

Keyphrases

circulating tumor
cell free
single molecule
nucleic acid
binding protein
transcription factor
induced apoptosis
cell cycle arrest
circulating tumor cells
oxidative stress
endoplasmic reticulum stress