HA/PEI-coated acridine orange-loaded gold-core silica shell nanorods for cancer-targeted photothermal and chemotherapy.
Carolina F RodriguesNatanael F FernandesDuarte de Melo-DiogoPaula C N FerreiraIlídio Joaquim CorreiaAndré Ferreira MoreiraPublished in: Nanomedicine (London, England) (2021)
Aims: To develop a tumor-targeted chemo-photothermal nanomedicine through the functionalization of acridine orange (AO)-loaded gold-core mesoporous silica shell (AuMSS) nanorods with polyethylenimine (PEI) and hyaluronic acid (HA). Methods: Functionalization of the AuMSS nanorods was achieved through the chemical linkage of PEI followed by electrostatic adsorption of HA. Results: HA functionalization improved AuMSS' cytocompatibility by decreasing blood hemolysis, and PEI-HA inclusion promoted a controlled and sustained AO release. In vitro assays revealed that HA functionalization increased the internalization of nanoparticles by human negroid cervix epithelioid carcinoma cancer (HeLa) cells, and the combinatorial treatment mediated by AuMSS/PEI/HA_AO nanorods presented an enhanced effect, with >95% of cellular death. Conclusion: AuMSS/PEI/HA_AO formulations can act as tumor-targeted chemo-photothermal nanomedicines for the combinatorial therapy of cervical cancer.
Keyphrases
- cancer therapy
- drug delivery
- photodynamic therapy
- hyaluronic acid
- papillary thyroid
- drug release
- cell cycle arrest
- locally advanced
- cell death
- cell proliferation
- gold nanoparticles
- signaling pathway
- genome wide
- high throughput
- dna methylation
- mesenchymal stem cells
- human immunodeficiency virus
- smoking cessation
- antiretroviral therapy
- chemotherapy induced
- walled carbon nanotubes