Human papillomavirus DNA in head and neck squamous cell carcinomas in the Free State, South Africa.
Atang BulaneDominique GoedhalsRiaz Y SeedatJacqueline GoedhalsFelicity BurtPublished in: Journal of medical virology (2020)
Human papillomaviruses (HPVs) have been associated with a subset of head and neck squamous cell carcinomas (HNSCCs). The aim of this study was to determine the prevalence of HPV DNA in archived formalin-fixed paraffin-embedded tissue from patients with histologically confirmed HNSCCs in a South African cohort. A nested PCR was used for the detection of HPV DNA targeting the L1 gene. Positive samples were confirmed using an in-house hemi-nested PCR targeting the E6 gene and genotyped by sequence determination of amplicons. HPV DNA was detected in 57/780 (7.3%) samples, with the highest prevalence being in the sinonasal tract (16.0%) and oropharynx (10.8%). HPV16 was the most frequently detected type, being found in 26/57 (45.6%) positive samples. The prevalence of HPV DNA in HNSCCs found in this study was lower than that found in developed countries.
Keyphrases
- high grade
- circulating tumor
- squamous cell
- cell free
- single molecule
- south africa
- risk factors
- nucleic acid
- endothelial cells
- genome wide
- cervical cancer screening
- cancer therapy
- real time pcr
- circulating tumor cells
- hiv positive
- human immunodeficiency virus
- hepatitis c virus
- sensitive detection
- amino acid
- solid phase extraction