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Analysis of Antibody Inhibition of PfEMP1 Binding by Competition ELISA.

Rebecca Wendelboe OlsenJennifer SuurbaarAnja Ramstedt Jensen
Published in: Methods in molecular biology (Clifton, N.J.) (2022)
Plasmodium falciparum express variant antigens on the surface of infected erythrocytes (IEs). These include proteins of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. PfEMP1 proteins mediate binding of IEs to various human endothelial receptors and induce antibodies response during natural infections. In this chapter, we describe a competition ELISA that is an assay for antibodies neutralizing binding of PfEMP1 proteins to their cognate receptor.
Keyphrases
  • plasmodium falciparum
  • endothelial cells
  • binding protein
  • dna binding
  • high throughput
  • heat shock
  • induced pluripotent stem cells
  • transcription factor
  • oxidative stress
  • dengue virus
  • single cell
  • heat stress