Multifunctional Near-Infrared Fluorescent Probes with Different Ring-Structure Trigger Groups for Cell Health Monitoring and In Vivo Esterase Activity Detection.
Jiemin WangZhidong TengLiang ZhangYuexia YangJing QianTing CaoYuping CaoWenwu QinYun LiuHuichen GuoPublished in: ACS sensors (2020)
A series of multifunctional ratiometric near-infrared fluorescent probes (CYOH-3, CYOH-4, CYOH-5, and CYOH-6) for esterase detection are designed by gradually changing the deflection of the plane twist in the molecule. These probes are composed of different ring-structure trigger groups (from three-membered ring to six-membered ring) and the same luminescent group CYOH. These probes show maximum absorption at ∼585 nm and a fluorescence emission peak at ∼655 ± 5 nm. In the presence of esterase, the probes were hydrolyzed to expose the fluorophore CYOH (λabs = 690 nm, λem = 710 ± 5 nm), thus exhibiting ratiometric near-infrared fluorescence. The probe CYOH-6 has lower plane deflection angle and better ratiometric (R = I710±5nm/I657±4nm) fluorescence properties than probes CYOH-3, CYOH-4, and CYOH-5. CYOH-6 (six-membered ring) has been successfully used to target esterase in mitochondria and distinguish between dead cells (esterase inactivation) and live cells. In addition, CYOH-6 has been well used for monitoring of esterase activity in zebrafish and mice, which proves that these probes have good prospects for clinical biomedical applications.
Keyphrases
- living cells
- fluorescent probe
- single molecule
- photodynamic therapy
- fluorescence imaging
- quantum dots
- induced apoptosis
- small molecule
- energy transfer
- cell cycle arrest
- drug delivery
- light emitting
- sensitive detection
- nitric oxide
- epithelial mesenchymal transition
- label free
- risk assessment
- nucleic acid
- endoplasmic reticulum stress
- mesenchymal stem cells
- reactive oxygen species
- adipose tissue
- health information
- high resolution
- human health