Diacylglycerol kinase δ functions as a proliferation suppressor in pancreatic β-cells.
Taiji SatoChihiro IshiwatariYukiko K KanekoYoko IshikawaYuki KimuraNaoya WatanabeIkumi AoshimaYukari MatsudaTakahiro NakayamaRina ChibaTakahiro FujinukiKai IwataQiang LuTakako UsukiFumio SakaneTomohisa IshikawaPublished in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021)
Although an aberrant reduction in pancreatic β-cell mass contributes to the pathogenesis of diabetes, the mechanism underlying the regulation of β-cell mass is poorly understood. Here, we show that diacylglycerol kinase δ (DGKδ) is a key enzyme in the regulation of β-cell mass. DGKδ expression was detected in the nucleus of β-cells. We developed β-cell-specific DGKδ knockout (βDGKδ KO) mice, which showed lower blood glucose, higher plasma insulin levels, and better glucose tolerance compared to control mice. Moreover, an increased number of small islets and Ki-67-positive islet cells, as well as elevated cyclin B1 expression in the islets, were detected in the pancreas of βDGKδ KO mice. DGKδ knockdown in the β-cell line MIN6 induced significant increases in bromodeoxyuridine (BrdU) incorporation and cyclin B1 expression. Finally, we confirmed that streptozotocin-induced hyperglycemia and β-cell loss were alleviated in βDGKδ KO mice. Thus, suppressing the expression or enzymatic activity of DGKδ that functions as a suppressor of β-cell proliferation could be a novel therapeutic approach to increase β-cell mass for the treatment of diabetes.
Keyphrases
- single cell
- cell therapy
- induced apoptosis
- cell proliferation
- cell cycle arrest
- cardiovascular disease
- squamous cell carcinoma
- glycemic control
- diabetic rats
- signaling pathway
- oxidative stress
- endoplasmic reticulum stress
- nitric oxide
- mesenchymal stem cells
- high glucose
- bone marrow
- diabetic nephropathy
- pi k akt
- hydrogen peroxide
- stress induced
- wild type