Yolk-shell nanostructured Fe3O4@C magnetic nanoparticles with enhanced peroxidase-like activity for label-free colorimetric detection of H2O2 and glucose.
Na LuMin ZhangLei DingJing ZhengCaixia ZengYanli WenGang LiuAli AldalbahiJiye ShiShiping SongXiaolei ZuoLihua WangPublished in: Nanoscale (2018)
Herein, we have developed a simple and facile method to synthesize yolk-shell nanostructured Fe3O4@C nanoparticles (NPs) as a multifunctional biosensing platform for the label-free colorimetric detection of H2O2 and glucose. It was demonstrated that Fe3O4@C yolk-shell nanostructures (YSNs) retained the magnetic properties that can be used for separation and concentration. Also importantly, the Fe3O4@C YSNs exhibited an intrinsic peroxidase-like activity that could quickly catalyze the enzyme substrate in the presence of H2O2 and produce a blue color. Compared to other similar ferric oxide-based NPs with different structures, Fe3O4@C YSNs exhibited greatly enhanced catalytic activities due to their unique structural features. Moreover, steady-state kinetics indicated the catalytic behaviors in agreement with the classic Michaelis-Menten models. Taking advantage of the high catalytic activity, Fe3O4@C YSNs were employed as novel peroxidase mimetics for label-free, rapid, sensitive, and specific colorimetric sensing of H2O2 and glucose, suggesting that Fe3O4@C YSNs have the potential for construction of portable sensors in the application of point-of-care (POC) diagnosis and on-site tests.
Keyphrases
- label free
- hydrogen peroxide
- magnetic nanoparticles
- gold nanoparticles
- blood glucose
- nitric oxide
- drug delivery
- fluorescent probe
- high resolution
- loop mediated isothermal amplification
- sensitive detection
- low cost
- living cells
- quantum dots
- oxide nanoparticles
- type diabetes
- mass spectrometry
- liquid chromatography
- insulin resistance
- crystal structure
- metabolic syndrome
- reduced graphene oxide
- molecularly imprinted
- climate change
- amino acid
- single cell