Point-of-Care Detection of Salivary Nitrite Based on the Surface Plasmon-Assisted Catalytic Coupling Reaction of Aromatic Amines.
Chen ZhaoRuyi ShiJiale WuXuan LuoXiangjiang LiuPublished in: Biosensors (2021)
Rapid quantification of nitrite (NO2-) in food, drink and body fluids is of significant importance for both food safety and point-of-care (POA) applications. However, conventional nitrite analytical methods are complicated, constrained to sample content, and time-consuming. Inspired by a nitrite-triggered surface plasmon-assisted catalysis (SPAC) reaction, a rapid point-of-care detection salivary nitrate was developed in this work. NO2- ions can trigger the rapid conversion of p-aminothiophenol (PATP) to p,p'-dimercaptozaobenzene (DMAB) on gold nanoparticles (GNPs) under light illumination, and the emerged new bands at ca. 1140, 1390, 1432 cm-1 originating from DMAB can be used to the quantification of nitrite. Meanwhile, to make the method entirely suitable for on-site fast screen or point-of-care application, the technique is needed to be further optimized. The calibration graph for nitrates was linear in the range of 1-100 µM with a correlation coefficient of 0.9579. The limit of detection was 1 µM. The facile method could lead to a further understanding of the progression and treatment of periodontitis and to guide professionals in planning on-site campaigns to effectively control periodontal diseases.
Keyphrases
- loop mediated isothermal amplification
- nitric oxide
- gold nanoparticles
- sensitive detection
- real time pcr
- label free
- quantum dots
- reduced graphene oxide
- magnetic resonance imaging
- risk assessment
- human health
- computed tomography
- room temperature
- drinking water
- machine learning
- deep learning
- single cell
- mass spectrometry
- energy transfer
- convolutional neural network
- highly efficient
- smoking cessation
- electron transfer
- neural network