Construction of à la carte QconCAT protein standards for multiplexed quantification of user-specified target proteins.
James JohnsonVictoria M HarmanCatarina FrancoEdward EmmottNichola RockliffeYaqi SunLu-Ning LiuAyako TakemoriNobuaki TakemoriRobert J BeynonPublished in: BMC biology (2021)
We refer to this approach as the ALACAT strategy as it permits à la carte design of quantification standards. ALACAT methodology is a major gain in flexibility of QconCAT implementation as it supports rapid editing and improvement of QconCATs and permits, for example, substitution of one peptide by another.