Mesenchymal cells and fluid flow stimulation synergistically regulate the kinetics of corneal epithelial cells at the air-liquid interface.

Kosuke KawataShigehisa AokiMaki FutamataMihoko Yamamoto-RikitakeIsao NakaoHiroshi EnaidaShuji Toda

Published in: Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie (2019)

The cell-cell interaction and fluid flow stimulation in the air-liquid interface synergistically or independently regulated the behavior of HCE-T cells. Fluid flow accelerated the phenotypic change from corneal fibroblasts and NIH 3T3 cells to myofibroblasts. Elucidation of the multicomponent interplay in this microenvironment will be critical to the homeostasis and regeneration of the cornea and other ocular tissues.

Keyphrases

stem cells
single cell
cell therapy
induced apoptosis
wound healing
gene expression
optical coherence tomography
ionic liquid
extracellular matrix
cell proliferation
optic nerve