Integration of 3D Fluorescence Imaging and Luminescent Thermometry with Core-Shell Engineered NaYF 4 :Nd 3+ /Yb 3+ /Ho 3+ Nanoparticles.

The design of rare-earth-doped upconversion/downshifting nanoparticles (NPs) for theoretical use in nanomedicine has garnered considerable interest. Previous research has emphasized luminescent nanothermometry and photothermal therapy, while three-dimensional (3D) near-infrared (NIR) luminescent tracers have received less attention. Our study introduces Nd 3+ -, Yb 3+ -, and Ho 3+ -doped NaYF 4 core-shell luminescent NPs as potential multiparametric nanothermometers and NIR imaging tracers. Nd 3+ sensitizes at 804 nm, while Yb 3+ bridges to activators Ho 3+ . We evaluated the photoluminescence properties of Nd 3+ -, Yb 3+ -, and Ho 3+ -doped core and core-shell NPs synthesized via polyol-mediated and thermal decomposition methods. The NaYF 4 :NdYbHo(7/15/3%)@NaYF 4 :Nd(15%) core-shell NPs demonstrate competitive nanothermometry capabilities. Specifically, the polyol-synthesized sample exhibits a sensitivity of 0.27% K -1 at 313 K (40 °C), whereas the thermally decomposed synthesized sample shows a significantly higher sensitivity of 0.55% K -1 at 313 K (40 °C) in the near-infrared range. Control samples indicate back energy transfer processes from both Yb and Ho to Nd, while Yb to Ho energy transfer enhances Ho 3+ -driven upconversion transitions in green and red wavelengths, suggesting promise for photodynamic therapy. Fluorescence molecular tomography confirms 3D NIR fluorescence nanoparticle localization in a biological media after injection, highlighting the potential of core-shell NPs as NIR luminescent tracers. The strategy's clinical impact lies in photothermal treatment planning, leveraging core-shell NPs for (pre)clinical applications, and enabling the easy addition of new functionalities through distinct ion doping.