Genome Mining of Streptomyces atratus SCSIO ZH16: Discovery of Atratumycin and Identification of Its Biosynthetic Gene Cluster.
Changli SunZhijie YangChunyan ZhangZhiyong LiuJianqiao HeQing LiuTianyu ZhangJianhua JuJunying MaPublished in: Organic letters (2019)
Genome mining of the deep sea-derived Streptomyces atratus SCSIO ZH16 enabled the activation of a cyclodepsipeptide gene cluster and isolation of its cinnamic acid-bearing product, atratumycin (1). Atratumycin's structure was elucidated on the basis of extensive spectroscopic experiments, X-ray data, and Marfey's method; a plausible biosynthesis and tailoring modification of 1 are also proposed and investigated. Additionally, atratumycin is active against Mycobacteria tuberculosis H37Ra and H37Rv with MICs of 3.8 and 14.6 μM, respectively.
Keyphrases
- genome wide
- mycobacterium tuberculosis
- copy number
- dna methylation
- genome wide identification
- rheumatoid arthritis
- small molecule
- high resolution
- molecular docking
- electronic health record
- high throughput
- big data
- hiv aids
- magnetic resonance imaging
- machine learning
- systemic lupus erythematosus
- transcription factor
- disease activity
- gene expression
- genome wide analysis
- computed tomography
- hepatitis c virus
- dual energy
- systemic sclerosis
- idiopathic pulmonary fibrosis