Short-term evaluation of photobiomodulation therapy on the proliferation and undifferentiated status of dental pulp stem cells.
L S FerreiraI M A DinizC M S MarandubaS P H MiyagiM F S D RodriguesC Moura-NettoMárcia Martins MarquesPublished in: Lasers in medical science (2018)
The aim of this in vitro study was to analyze the effect of photobiomodulation therapy (PBMT) on the proliferation and undifferentiating status of stem cell from human exfoliated deciduous teeth (SHEDs). PBMT was carried out with an aluminum gallium indium phosphide (InGaAlP) diode laser in contact and punctual mode (continuous wave, 660 nm, 20 mW, 0.028 cm2, and average energy densities of 1 (1 s), 3 (4 s), 5 (7 s), 10 (14 s), 15 (21 s), or 20 (28 s) J/cm2 per point). The immunoprofile of the SHEDs was analyzed using flow cytometry. Cell proliferation was assessed by the MTT reduction assay. Gene expressions of mesenchymal stem cell markers (OCT4, Nestin, CD90, and CD105) were assessed by RT-qPCR 48 h after PBMT. Data were compared by analysis of variance (ANOVA) and Tukey's test (p ≤ 0.05). Cells cultured under nutritional deficit and treated with PBMT at 5 J/cm2 presented similar cell growth than those of positive control group. Cell growth was significantly higher than those of other groups. Mesenchymal stem cell gene markers were still expressed after PBMT at 5 J/cm2. In a short-term analysis, PBMT increases the number of stem cells with no interference in the undifferentiated state of the irradiated cells, which opens wide possibilities for application in tissue regeneration.
Keyphrases
- stem cells
- induced apoptosis
- cell therapy
- mesenchymal stem cells
- flow cytometry
- cell proliferation
- endothelial cells
- cell cycle arrest
- signaling pathway
- bone marrow
- copy number
- oxidative stress
- cell death
- endoplasmic reticulum stress
- wound healing
- big data
- machine learning
- cell cycle
- dna methylation
- high resolution
- single cell
- artificial intelligence