Accuracy of a rapid multiplex PCR plus a chromogenic phenotypic test algorithm for detection of ESBL and carbapenemase-producing Gram-negative bacilli in positive blood culture bottles.
Sean Wei Xiang OngPei Yun HonSharon Syn Hui WeeJonathan Wei Zhong ChiaShehara MendisEzlyn IzharuddinRay Junhao LinPo Ying ChiaRees Chin Swee SimMark I-Cheng ChenAngela ChowJoanne YoongDavid Chien LyeChristine B TengPaul Anantharajah TambyahRitu BanerjeeRobin PatelPartha Pratim DeShawn VasooPublished in: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America (2021)
We studied the performance of an algorithm combining multiplex-PCR with phenotypic detection of ESBLs and carbapenemases directly from positive blood culture bottles in patients with Gram-negative bacteremia and found good concordance with routine cultures. Such an algorithm may be a tool to improve time-to-optimal therapy in patients with Gram-negative bacteremia.