Intracellular Conformation of Amyotrophic Lateral Sclerosis-Causative TDP-43.
Masataka KinjoSachiko YunoRintaro KawamuraMasataka KinjoPublished in: International journal of molecular sciences (2023)
Transactive response element DNA/RNA-binding protein 43 kDa (TDP-43) is the causative protein of amyotrophic lateral sclerosis (ALS); several ALS-associated mutants of TDP-43 have been identified. TDP-43 has several domains: an N-terminal domain, two RNA/DNA-recognition motifs, and a C-terminal intrinsically disordered region (IDR). Its structures have been partially determined, but the whole structure remains elusive. In this study, we investigate the possible end-to-end distance between the N- and C-termini of TDP-43, its alterations due to ALS-associated mutations in the IDR, and its apparent molecular shape in live cells using Förster resonance energy transfer (FRET) and fluorescence correlation spectroscopy (FCS). Furthermore, the interaction between ALS-associated TDP-43 and heteronuclear ribonucleoprotein A1 (hnRNP A1) is slightly stronger than that of wild-type TDP-43. Our findings provide insights into the structure of wild-type and ALS-associated mutants of TDP-43 in a cell.
Keyphrases
- amyotrophic lateral sclerosis
- energy transfer
- wild type
- single molecule
- quantum dots
- binding protein
- induced apoptosis
- single cell
- circulating tumor
- bone marrow
- nucleic acid
- molecular dynamics simulations
- stem cells
- mesenchymal stem cells
- cell proliferation
- magnetic resonance imaging
- oxidative stress
- heat shock protein