In vitro comparison of viral replication and cytopathology induced by SARS-CoV-2 variants.
Kruttika S PhadkeNathaniel B A HigdonBryan H BellairePublished in: Access microbiology (2024)
A myriad of coronaviruses cause diseases from a common cold to severe lung infections and pneumonia. SARS-CoV-2 was discovered to be the etiologic agent of the Coronavirus pandemic and many laboratory techniques were examined for virus culture and basic and applied research. Understanding the replication kinetics and characterizing the effect the virus has on different cell lines is crucial for developing in vitro studies. With the emergence of multiple variants of SARS-CoV-2, a comparison between their infectivity and replication in common cell lines will help give us a clear understanding of their characteristic differences in pathogenicity. In this study we compared the cytopathic effect and replication of Wild-Type (USA/WA1), Omicron (B.1.1.529), and Delta (B.1.617.2) variants on five different cell lines; VeroE6, VeroE6 cells expressing high endogenous ACE2, VeroE6 cells expressing human ACE2 and TMPRSS2, Calu3 cells highly expressing human ACE2 and A549 cells. This data will aid researchers with experimental planning and viral pathogenicity analysis and provide a baseline for testing any future variants.
Keyphrases
- sars cov
- induced apoptosis
- cell cycle arrest
- respiratory syndrome coronavirus
- endothelial cells
- copy number
- wild type
- endoplasmic reticulum stress
- cell death
- intensive care unit
- oxidative stress
- angiotensin converting enzyme
- cell proliferation
- early onset
- coronavirus disease
- machine learning
- escherichia coli
- cystic fibrosis
- mechanical ventilation
- gene expression
- dna methylation
- deep learning
- pseudomonas aeruginosa
- big data
- drug induced
- current status