AutoCloner: automatic homologue-specific primer design for full-gene cloning in polyploids.
Alexander CoultonKeith J EdwardsPublished in: BMC bioinformatics (2020)
AutoCloner is the first tool to fully-automate primer design for gene cloning in polyploids, where previously the consensus within the wheat community was to perform this process manually. The web interface for AutoCloner provides a simple and effective polyploid primer-design method for gene cloning, with no need for researchers to download software or input any other details other than their sequence of interest.