Evaluation of full-length nanopore 16S sequencing for detection of pathogens in microbial keratitis.

Liying LowPablo Fuentes-UtrillaJames HodsonJohn D O'NeilAmanda E RossiterGhazala BegumKusy Suleiman Philip Ian MurrayGraham R WallaceNicholas J LomanSaaeha Rauznull null

Published in: PeerJ (2021)

We have optimised collection methods and demonstrated a novel workflow for identification of bacterial microbial keratitis using full-length 16S nanopore sequencing.

Keyphrases

single molecule
microbial community
single cell
solid state
gram negative
loop mediated isothermal amplification
electronic health record
real time pcr
label free
bioinformatics analysis
quantum dots