Functional analysis of isoprenoid precursors biosynthesis by quantitative metabolomics and isotopologue profiling.

Sara Castaño-CerezoHanna Kulyk-BarbierPierre MillardJean-Charles PortaisStéphanie HeuxGilles Truan Floriant Bellvert

Published in: Metabolomics : Official journal of the Metabolomic Society (2019)

This methodology fills one of the last technical gaps for functional studies of isoprenoids biosynthesis and should be applicable to other eukaryotic and prokaryotic (micro)organisms after adaptation of some organism-dependent steps. This methodology also opens the way to 13C-metabolic flux analysis of isoprenoid biosynthesis.

Keyphrases

cell wall
mass spectrometry
high resolution
single cell
gram negative
case control