Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility.
Yoshitaka KanaokaDaichi KuniyoshiEri InadaYohei KoideYoshihiro OkamotoHideshi YasuiYuji KishimaPublished in: Plant methods (2018)
We demonstrated that AC to generate callus populations derived from immature microspores is a useful methodology for genetic study. The callus population facilitated detection of TRD at multiple HS loci and dramatically shortened the process for mapping hybrid sterility genes.