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Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility.

Yoshitaka KanaokaDaichi KuniyoshiEri InadaYohei KoideYoshihiro OkamotoHideshi YasuiYuji Kishima
Published in: Plant methods (2018)
We demonstrated that AC to generate callus populations derived from immature microspores is a useful methodology for genetic study. The callus population facilitated detection of TRD at multiple HS loci and dramatically shortened the process for mapping hybrid sterility genes.
Keyphrases
  • genome wide
  • copy number
  • gene expression
  • mass spectrometry
  • transcription factor