Literature-Based Drug Repurposing in Traditional Chinese Medicine: Reduced Inflammatory M1 Macrophage Polarization by Jisil Haebaek Gyeji-Tang Alleviates Cardiovascular Disease In Vitro and Ex Vivo.
Ga-Ram YuSeung-Jun LeeDa-Hoon KimDong-Woo LimHyuck KimWon-Hwan ParkJai-Eun KimPublished in: Evidence-based complementary and alternative medicine : eCAM (2020)
Relatively high proportions of proinflammatory M1-like macrophages in tissues may lead to vascular impairment and trigger numerous diseases including atherosclerosis-related cardiovascular disease (CVD). Jisil Haebaek Gyeji-tang (JHGT), a polyherbal decoction, is traditionally used to treat various human ailments including chest pain, angina, and myocardial infarction. In the present study, we investigated the anti-inflammatory effects of JHGT on lipopolysaccharide- (LPS-) stimulated M1 macrophage polarization generated via the mitogen-activated protein kinases (MAPKs) pathway in RAW 264.7 mouse macrophages. The reducing power of JHGT was also investigated using DAF-FA DA in a zebrafish model. JHGT significantly reduced inflammatory mediator levels, including iNOS, COX2, TNF-α, IL-6, and IL-1β, as compared with LPS-stimulated controls in vitro and ex vivo. Furthermore, JHGT suppressed the ERK1/2, JNK, and p38 MAPK pathways and reduced p-IκBα levels and the nuclear translocation of NF-κB in RAW 264.7 cells. In addition, treatment with JHGT significantly reduced the NO levels in LPS-treated zebrafish larva ex vivo. Our findings show the potent anti-inflammatory properties of JHGT are due to its suppression of MAPK signaling, NF-κB translocation, and M1 macrophage polarization.
Keyphrases
- anti inflammatory
- signaling pathway
- cardiovascular disease
- induced apoptosis
- oxidative stress
- inflammatory response
- pi k akt
- lps induced
- endothelial cells
- rheumatoid arthritis
- systematic review
- cell cycle arrest
- gene expression
- type diabetes
- coronary artery disease
- cell proliferation
- cardiovascular events
- left ventricular
- metabolic syndrome
- toll like receptor
- coronary artery
- induced pluripotent stem cells
- mouse model