Using HPLC for the determination of platinum drugs in biological matrixes after derivatization with diethyldithiocarbamate.
Dorota TuroňováLenka Kujovska KrcmovaMilan VošmikBohuslav MelicharFrantišek ŠvecPublished in: Journal of separation science (2023)
Challenges and pitfalls in the application of diethyldithiocarbamate derivatization for LC analysis of cisplatin and oxaliplatin, as well as the suitability of this method for different biological matrices with implications for use in routine practice have been identified. The LC of platinum drugs presents a significant challenge. They are polar compounds with poor retention on reverse phase packings. Cisplatin also exhibits poor absorption in UV and ionization in mass spectrometry. Therefore, we developed and optimized a derivatization approach for the LC analysis of total platinum in plasma, plasma ultrafiltrate, peritoneal fluid, and urine. Derivatization in urine proved to be difficult due to the complexity of the matrix, and extended testing was required. Our results highlight the important issues affecting the efficiency, reliability, and suitability of platinum drug derivatization. Although precolumn derivatization is less selective than its postcolumn counterpart, the application of precolumn derivatization is a simple, rapid, and universal approach for the determination of platinum drugs by HPLC. One of its major advantages is that it allows a more affordable analysis using UV detection without the need for additional high-end instrumentation such as a MS detector.
Keyphrases
- solid phase extraction
- simultaneous determination
- high performance liquid chromatography
- ms ms
- mass spectrometry
- liquid chromatography
- gas chromatography
- liquid chromatography tandem mass spectrometry
- tandem mass spectrometry
- gas chromatography mass spectrometry
- ultra high performance liquid chromatography
- molecularly imprinted
- high resolution mass spectrometry
- high resolution
- capillary electrophoresis
- multiple sclerosis
- computed tomography
- magnetic resonance imaging
- primary care
- aqueous solution
- magnetic resonance