Autophagy in hematopoietic stem cell development of the embryo.
Yumin LiuSifan LuoYuehang ChenZhuan LiPublished in: Autophagy (2024)
Hematopoietic stem cells (HSC) emerge from hemogenic endothelial cells (HEC) in the aorta-gonad-mesonephros (AGM) region of embryos, which go through the pre-HSC process. Various intrinsic and extrinsic factors are involved in this process. We recently discovered that the existence of distinct macroautophagic/autophagic statuses in hematopoietic precursors is related to the hematopoietic potential of pre-HSCs and the depletion of the Atg5 (autophagy related 5) gene specifically in endothelial cells impaired in the transition of endothelial to pre-HSCs, by hampering the autophagic process, likely via the NCL (nucleolin) pathway. Abbreviation: Atg5: autophagy related 5; EGFP: enhanced green fluorescent protein; EHT: endothelial-to-hematopoietic transition; HEC: hemogenic endothelial cell; HSC: hematopoietic stem cell; NCL: nucleolin; RFP: red fluorescent protein.
Keyphrases
- endothelial cells
- hematopoietic stem cell
- cell death
- cell cycle arrest
- endoplasmic reticulum stress
- induced apoptosis
- bone marrow
- high glucose
- signaling pathway
- quantum dots
- oxidative stress
- vascular endothelial growth factor
- living cells
- amino acid
- binding protein
- label free
- genome wide
- dna methylation
- gene expression
- cell proliferation
- pregnant women
- drug induced
- genome wide analysis
- aortic dissection
- pi k akt