Mastadenovirus Molecular Diversity in Waste and Environmental Waters from the Lisbon Metropolitan Area.
Joana CavadasRicardo ParreiraInês Carvalho LeonardoMaria Teresa Barreto CrespoMónica NunesPublished in: Microorganisms (2022)
In face of the absence of epidemiological data regarding the circulation of human adenoviruses (HAdV) in Portugal, this study aimed at the evaluation of their molecular diversity in waste and environmental waters in the Lisbon Metropolitan Area (LMA). Using samples collected between 2018 and 2021, the HAdV hexon protein-coding sequence was partially amplified using three nested touch-down PCR protocols. The amplification products obtained were analyzed in parallel by two approaches: molecular cloning followed by Sanger sequencing and Next-Generation Sequencing (NGS) using Illumina ® sequencing. The analysis of NGS-generated data allowed the identification of a higher diversity of HAdV-A (19%), -B (1%), -C (3%), -D (24%), and -F (25%) viral types, along with murine adenovirus (MAdV-2; 30%) in the wastewater treatment plant samples. On the other hand, HAdV-A (19%), -D (32%), and -F (36%) were identified in environmental samples, and possibly MAdV-2 (14%). These results demonstrate the presence of fecal contamination in environmental waters and the assessment of the diversity of this virus provides important information regarding the distribution of HAdV in LMA, including the detection of HAdV-F41, the most frequently reported in water worldwide.
Keyphrases
- wastewater treatment
- life cycle
- human health
- risk assessment
- electronic health record
- endothelial cells
- sars cov
- heavy metals
- single cell
- antibiotic resistance genes
- healthcare
- municipal solid waste
- label free
- climate change
- gene expression
- microbial community
- small molecule
- sewage sludge
- binding protein
- health information
- cell wall